precision plus proteintm dual colour standards Search Results


molecular weight markers  (Bio-Rad)
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precision plus proteintm standard  (Bio-Rad)
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Bio-Rad precision plus proteintm standard
Precision Plus Proteintm Standard, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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precision plus proteintm dual color standards  (Bio-Rad)
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Bio-Rad precision plus proteintm dual color standards
Precision Plus Proteintm Dual Color Standards, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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precision plus proteintm unstained protein standard  (Bio-Rad)
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precision plus proteintm westernctm blotting standards  (Bio-Rad)
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Bio-Rad precision plus proteintm westernctm blotting standards
Precision Plus Proteintm Westernctm Blotting Standards, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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precision plus proteintm kaleidoscopetm prestained protein standards  (Bio-Rad)
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Bio-Rad precision plus proteintm kaleidoscopetm prestained protein standards
Precision Plus Proteintm Kaleidoscopetm Prestained Protein Standards, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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precision plus proteintm all blue standard  (Bio-Rad)
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Bio-Rad precision plus proteintm all blue standard
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precision plus proteintm dual xtra  (Bio-Rad)
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Bio-Rad precision plus proteintm dual xtra
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kaleidoscope  (Bio-Rad)
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Bio-Rad kaleidoscope
Figure 2: Western blotting detection of GnRH-I fusion protein obtained from transfected COS 1 cells and labelled with anti- GnRH-I (left) and anti V5 antibodies (right). The first [10], second [6] and the new vaccines were used to transfect COS 1 cells. Lane 1 shows the <t>Kaleidoscope</t> ladder, lanes 2, 3 and 4 containing transfected COS1 cell lysate of the first, second and new vaccines respectively. Lanes 6, 7 and 8 containing transfected COS1 cell culture supernatant of the first, second and new vaccine repectively. Lane 5 and 9 containing non-transfected COS1 cell lysate and culture supernatant respectively (negative control) and lane 10 lacking both anti-GnRH-I (left) and anti V5 antibodies (right) in the Western blotting detection of the fusion protein. The newly engineered vaccine produce fusion protein (35.216 KD) detected both in cell lysate (lane 4) and cell culture supernatant (lane 8). GnRH-I fusion protein (17.138 KD) of the first vaccine (lane 2) was detected in the cell lysate (lane 6). GnRH-I fusion protein (18.871 KD) of the second vaccine was detected both in cell lysate (lane 3) and cell culture supernatant (lane 7). GnRH-I fusion protein was not detected in any of the control lane 5, 9 and 10.
Kaleidoscope, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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precision plus proteintm all blue prestained protein standards  (Bio-Rad)
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Bio-Rad precision plus proteintm all blue prestained protein standards
Figure 2: Western blotting detection of GnRH-I fusion protein obtained from transfected COS 1 cells and labelled with anti- GnRH-I (left) and anti V5 antibodies (right). The first [10], second [6] and the new vaccines were used to transfect COS 1 cells. Lane 1 shows the <t>Kaleidoscope</t> ladder, lanes 2, 3 and 4 containing transfected COS1 cell lysate of the first, second and new vaccines respectively. Lanes 6, 7 and 8 containing transfected COS1 cell culture supernatant of the first, second and new vaccine repectively. Lane 5 and 9 containing non-transfected COS1 cell lysate and culture supernatant respectively (negative control) and lane 10 lacking both anti-GnRH-I (left) and anti V5 antibodies (right) in the Western blotting detection of the fusion protein. The newly engineered vaccine produce fusion protein (35.216 KD) detected both in cell lysate (lane 4) and cell culture supernatant (lane 8). GnRH-I fusion protein (17.138 KD) of the first vaccine (lane 2) was detected in the cell lysate (lane 6). GnRH-I fusion protein (18.871 KD) of the second vaccine was detected both in cell lysate (lane 3) and cell culture supernatant (lane 7). GnRH-I fusion protein was not detected in any of the control lane 5, 9 and 10.
Precision Plus Proteintm All Blue Prestained Protein Standards, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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precision plus proteintm unstained standard  (Bio-Rad)
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Bio-Rad precision plus proteintm unstained standard
Figure 2: Western blotting detection of GnRH-I fusion protein obtained from transfected COS 1 cells and labelled with anti- GnRH-I (left) and anti V5 antibodies (right). The first [10], second [6] and the new vaccines were used to transfect COS 1 cells. Lane 1 shows the <t>Kaleidoscope</t> ladder, lanes 2, 3 and 4 containing transfected COS1 cell lysate of the first, second and new vaccines respectively. Lanes 6, 7 and 8 containing transfected COS1 cell culture supernatant of the first, second and new vaccine repectively. Lane 5 and 9 containing non-transfected COS1 cell lysate and culture supernatant respectively (negative control) and lane 10 lacking both anti-GnRH-I (left) and anti V5 antibodies (right) in the Western blotting detection of the fusion protein. The newly engineered vaccine produce fusion protein (35.216 KD) detected both in cell lysate (lane 4) and cell culture supernatant (lane 8). GnRH-I fusion protein (17.138 KD) of the first vaccine (lane 2) was detected in the cell lysate (lane 6). GnRH-I fusion protein (18.871 KD) of the second vaccine was detected both in cell lysate (lane 3) and cell culture supernatant (lane 7). GnRH-I fusion protein was not detected in any of the control lane 5, 9 and 10.
Precision Plus Proteintm Unstained Standard, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sds page gels  (Bio-Rad)
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Bio-Rad sds page gels
Figure 2: Western blotting detection of GnRH-I fusion protein obtained from transfected COS 1 cells and labelled with anti- GnRH-I (left) and anti V5 antibodies (right). The first [10], second [6] and the new vaccines were used to transfect COS 1 cells. Lane 1 shows the <t>Kaleidoscope</t> ladder, lanes 2, 3 and 4 containing transfected COS1 cell lysate of the first, second and new vaccines respectively. Lanes 6, 7 and 8 containing transfected COS1 cell culture supernatant of the first, second and new vaccine repectively. Lane 5 and 9 containing non-transfected COS1 cell lysate and culture supernatant respectively (negative control) and lane 10 lacking both anti-GnRH-I (left) and anti V5 antibodies (right) in the Western blotting detection of the fusion protein. The newly engineered vaccine produce fusion protein (35.216 KD) detected both in cell lysate (lane 4) and cell culture supernatant (lane 8). GnRH-I fusion protein (17.138 KD) of the first vaccine (lane 2) was detected in the cell lysate (lane 6). GnRH-I fusion protein (18.871 KD) of the second vaccine was detected both in cell lysate (lane 3) and cell culture supernatant (lane 7). GnRH-I fusion protein was not detected in any of the control lane 5, 9 and 10.
Sds Page Gels, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Figure 2: Western blotting detection of GnRH-I fusion protein obtained from transfected COS 1 cells and labelled with anti- GnRH-I (left) and anti V5 antibodies (right). The first [10], second [6] and the new vaccines were used to transfect COS 1 cells. Lane 1 shows the Kaleidoscope ladder, lanes 2, 3 and 4 containing transfected COS1 cell lysate of the first, second and new vaccines respectively. Lanes 6, 7 and 8 containing transfected COS1 cell culture supernatant of the first, second and new vaccine repectively. Lane 5 and 9 containing non-transfected COS1 cell lysate and culture supernatant respectively (negative control) and lane 10 lacking both anti-GnRH-I (left) and anti V5 antibodies (right) in the Western blotting detection of the fusion protein. The newly engineered vaccine produce fusion protein (35.216 KD) detected both in cell lysate (lane 4) and cell culture supernatant (lane 8). GnRH-I fusion protein (17.138 KD) of the first vaccine (lane 2) was detected in the cell lysate (lane 6). GnRH-I fusion protein (18.871 KD) of the second vaccine was detected both in cell lysate (lane 3) and cell culture supernatant (lane 7). GnRH-I fusion protein was not detected in any of the control lane 5, 9 and 10.

Journal: Journal of Vaccines & Vaccination

Article Title: Immunization of Female Mice with a Plasmid DNA Vaccine Coding Eight Repeats of Gonadotrophin Releasing Hormone(Gnrh-I) and Eight T-Helper Epitopes Suppress Fertility In Vivo

doi: 10.4172/2157-7560.1000282

Figure Lengend Snippet: Figure 2: Western blotting detection of GnRH-I fusion protein obtained from transfected COS 1 cells and labelled with anti- GnRH-I (left) and anti V5 antibodies (right). The first [10], second [6] and the new vaccines were used to transfect COS 1 cells. Lane 1 shows the Kaleidoscope ladder, lanes 2, 3 and 4 containing transfected COS1 cell lysate of the first, second and new vaccines respectively. Lanes 6, 7 and 8 containing transfected COS1 cell culture supernatant of the first, second and new vaccine repectively. Lane 5 and 9 containing non-transfected COS1 cell lysate and culture supernatant respectively (negative control) and lane 10 lacking both anti-GnRH-I (left) and anti V5 antibodies (right) in the Western blotting detection of the fusion protein. The newly engineered vaccine produce fusion protein (35.216 KD) detected both in cell lysate (lane 4) and cell culture supernatant (lane 8). GnRH-I fusion protein (17.138 KD) of the first vaccine (lane 2) was detected in the cell lysate (lane 6). GnRH-I fusion protein (18.871 KD) of the second vaccine was detected both in cell lysate (lane 3) and cell culture supernatant (lane 7). GnRH-I fusion protein was not detected in any of the control lane 5, 9 and 10.

Article Snippet: A Kaleidoscope (Precision Plus ProteinTM BIO-RAD) protein marker was included in the starting wells and the separated protein bands in the gels were transferred to a nitrocellulose membrane (HybondTMECL membrane, Amersham Bioscience).

Techniques: Western Blot, Transfection, Vaccines, Cell Culture, Negative Control, Control